"The techniques we've highlighted so far are all clever manipulations of white light, a mixture of many visible wavelengths of light. But in fluorescence microscopy, instead of illuminating our sample with the mixture, we choose a specific wavelength of light. That light is called the excitation light because, well, it excites certain structures based on their chemistry. Those excitable structures then emit some light on their own. The emission light will then travel back through the objective, get past barriers that filter out the excitation light, and then show up in our eyes as vivid color. With our upgrade, we have four different wavelengths of light that we can use for fluorescence microscopy, and the parts of the sample that we see fluoresce will depend on which light we use."
Journey to the Microcosmos- We Upgraded Our Microscope... Again!
Images Originally Captured by Jam's Germs
