Jun 2, 2021
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Anya is live and ready to show you everything. Watch her strip, dance, and perform exclusive shows just for you. Interact in real-time and make your fantasies come true.
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You're going to fall in love and feel it sweep you away so deeply, and then you can just as suddenly fall out of love, so much so that it breaks you. And you'll make mistakes. You'll be so sure of yourself one moment and then out of your head the next. It's part of being human.
My dear cold blooded king (webtoon) - Lifelight
What is Cell Biology?
What is Cell Biology?
C O N T E N T S:
GENERAL INFO
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The South Dakota State University Department of Biology & Microbiology invites applications for a tenure-track Assistant Professor position in the area of Cell Biology with emphasis on mammalian models.(More…)
These investments include expanded capabilities in fluorescence and confocal microscopy, tissue-sectioning, laser capture microdissection, next…
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New Post has been published on Haarlem updates
Nieuw bericht op http://www.haarlemupdates.nl/2015/09/22/twee-keer-golfen-ten-bate-van-het-nieuwe-centrum-kindermisbruik/
Twee keer golfen ten bate van het nieuwe Centrum Kindermisbruik
17 oktober a.s. opent het eerste Multidisciplinair Centrum Kindermishandeling (MDCK) van Nederland haar deuren in het Spaarne Gasthuis te Hoofddorp. Op dit moment ontbreekt het nog aan voldoende middelen om dit unieke centrum op een kindvriendelijke manier in te richten. Tijdens het StreetGolf...
WTF, MCF10A??? how can you sit in trypsin for 30 bloody minutes and *still* not detach? honestly you are THE WORST. i thought MDCK were bad enough, well little did i know. well come to think of it, maybe they *were* the worst, because they would also require trypsin for about half an hour but they would look a bit damaged the next day, so i figured out my own protocol in the end. MCF10A though, i’m impressed, i’m very fucking impressed. literally 10 minutes after i add medium, they drop down, attach and begin spreading. no, you cannot pipette them up, they’re properly attached and all. basically the cell suspension that you needed for the experiment, is gone. WTF???
except *of course* when i need them to attach and spread, like i’ve been waiting for um, 7 hours, and they’re still kinda rounded? which is not what i need? oh okay, i get it, you don’t like bare greiner’s plastic (honestly i don’t blame you, i’m not particularly fond of it myself, but it’s all we’ve got so please work with me here? MCF10A? please?). but you can’t seriously think that will stop a cell biologist with a shitton of experience, right? you want matrix? i’ll give you matrix, i’ll seed you @300K, @500K if needs be, and then i will replate you into the same wells @50K for the experiment, ah? howaboutthat? oh and i’ll coat a couple of wells with fibronectin as well, just to cover all the bases. pfffft.
okay i need to change my workstuff tag to “angrycellbiology”. yes it’s a rip-off but idc.
oh hey, joan brugge also recommends trypsin for 30 min, heh. so i guess there is no way around it then. UGH.
ETA: fucci is go, fingers crossed.
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