#frap

Se Acabó (it's over)

¡Viva las Fuerzas Armadas de Liberación! a Mexican revolutionary song over EZLN footage.

Lyrics (the auto-generated subs in the video have some errors):

It’s back down to what used to be normal temps for us, in the 20’s (F) with wind chills in the teens.

So Frap has been back a bit more, especially during the day. She’s so cozy in her little heated house. 🥰

Serves: 2

* 1 cup strong coffee made ahead of time and very cold or 2 shots espresso cooled

* 1 cup milk of choice

* 2 cups ice

* ½ cup chocolate syrup - store bought or homemade

* ⅓ cup chocolate chips

* Toppings: homemade whipped cream and chocolate chips

Source: https://www.tiktok.com/t/ZTFLD61Bd/

3D Vision

You can’t fix a broken car without looking inside to see what’s going on with the engine. Understanding the inner workings of our cells is also a key step in establishing processes underpinning both healthy function and disease. A technique called fluorescence recovery after photobleaching (FRAP) visualises the movement of molecules within cells, showing the progression of fluorescently labelled molecules across a thin film. Cells in our bodies are not flat slivers, however, so a new approach combines FRAP with other microscopy approaches and simulations to produce 3-dimensional visualisations, such as the T-cells pictured, showing the flow of the structural protein actin along the cell surface. Watching and quantitatively measuring the movement of molecules within cells shows how fundamental processes occur, what changes in this natural dynamic when disease or malfunction sets in, and perhaps even how new drugs or treatments affect the internal goings on.

Written by Anthony Lewis

Video from work by Huw Colin-York and colleagues

MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK

Video originally published with a Creative Commons Attribution 4.0 International (CC BY 4.0)

Published in Small Methods, March 2022

Rad Coffee designs by Austin Pardun