#elisatechnique

There are many different ELISA assays available in the market, for researchers to choose from. Selecting the right type of ELISA assay for any given project can have a profound impact on the future success of that project. Some of the salient points that should be kept in mind, when selecting an ELISA assay, will be discussed in this article.

Catalog NO.：FY-EH4659

TRACP-5b(TartRate-resistant Acid Phosphatase 5b) Basic information：Tartrate-resistant acid phosphatase (TRAP or TRAPase), also called acid phosphatase 5, tartrate resistant (ACP5), is a glycosylated monomeric metalloprotein enzyme expressed in mammals. It has a molecular weight of approximately 35kDa, a basic isoelectric point (7.6–9.5), and optimal activity in acidic conditions. TRAP is synthesized as latent proenzyme and activated by proteolytic cleavage and reduction. It is differentiated from other mammalian acid phosphatases by its resistance to inhibition by tartrate and by its molecular weight.Under normal circumstances, TRAP is highly expressed by osteoclasts, activated macrophages, neurons, and by the porcine endometrium during pregnancy.

 In newborn rats, TRAP is also detectable in the spleen, thymus, liver, kidneys, skin, lung, and heart at low levels. TRAP expression is increased in certain pathological conditions. These include leukaemic reticuloendotheliosis (hairy cell leukaemia), Gaucher’s disease, HIV-induced encephalopathy, osteoclastoma and osteoporosis, and metabolic bone diseases.

Test method：

Feiyue’s Human TRACP-5b(TartRate-resistant Acid Phosphatase 5b) is an ELISA reagent for detection of MASP2(Mannan-binding lectin serine protease 2) in plasma.

This kit uses sandwich ELISA to detect the concentration of Human TRACP-5b(TartRate-resistant Acid Phosphatase 5b). Human TRACP-5b(TartRate-resistant Acid Phosphatase 5b)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human TRACP-5b(TartRate-resistant Acid Phosphatase 5b) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human TRACP-5b(TartRate-resistant Acid Phosphatase 5b) in the sample can be calculated by comparing the absorbance of the sample with the standard.

When researchers are working with a completely unknown sample, they use the sandwich ELISA technique to detect the presence of target antigens and measure their concentration in the sample. This is because the sandwich ELISA technique offers superior sensitivity and greater durability, which together make it an excellent diagnostic tool for medical processes. This technique is particularly useful in the process of testing for drugs and identifying food allergens. Sandwich ELISA is simply one of many options in this assay category. Some of the other types of ELISA assays include direct, indirect, and competitive ELISA. Among these, the sandwich ELISA technique is considered best for quantifying the antigens that are ‘sandwiched’ between detection antibody and capture antibody. In terms of sensitivity, sandwich ELISA is anywhere between two to five times superior to indirect, direct, and competitive ELISA. Hence, sandwich ELISA can be used to quickly and accurately detect antigens in unknown samples. In the sandwich ELISA technique, antigens don’t need to be purified before the measurement. Hence, it is ideal for complex samples and is also known for being very flexible. Usually, monoclonal and polyclonal antibodies are used for capture and detection in this technique.