Human Tau Protein (MAPT) ELISA kit
Catalog No:FY-EH6846 Size: 96
The kit is intended for use in quantitative determination of Human MAPT concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity: 18.75 pg/mL.
Detection Range: 31.2-2000 pg/mL.
No significant cross-reactivity or interference between Human MAPT and analogues was observed.
Repeatability: Coefficient of variation is < 10%.
Principle of the Procedure
This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Human MAPT. Standards or samples containing Human MAPT are added to the plate and reacted with capture antibody. A second anti-Human MAPT antibody labeled biotin is then added and binds to Human MAPT captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Human MAPT-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Human MAPT in the samples is then calculated from the OD value by establishing a standard curve.