Check the first post with Elementalmech headcanons...

Bfr s7

He never had a thought of being trans, since he strictly followed his programing

Later after s4 he never had time to think about it, since he was in Zane's head and seemed to help him 24/7.

Though he never enjoyed being used she/her to and feminine labels.

S7

He rebuild his body bc he wanted to be a separate person + urgent situation to safe Cyrus Borg. But he did adopt a masculine persona because he knew that Samurai X, even if Nya was its previous owner was always viewed as a man and decided to go with it, later realizing that he likes it.

After s7 bfr s8

After being in the news a bunch, having a time to grow into the Samurai X persona and being called a 'he' by society he actually realized that he likes that! But he was too nervous to say it to the ninja because they would for sure ask how he understood it and then he would need to reveal his X persona.

S8

Even though he was later revealed that he was Samurai X, he still didn't come out to anyone and started to be kinda unsure about it.

S9

He eventually came out to Nya and Skylor, despite it being not really a good moment... But they accepted him! They started to use he/him to him, but in that time he still had the name P.I.X.A.L/Pixal.

After s10

He eventually came out to all the ninja and started to use 'Pixel' to himself, because in his opinion it was sounded more masculine. Though, he felt really awkward and kinds not in his plate when called a man so others quickly let go of that and kept his old voice.

In between s11 n before s15

Everything was relative the same.

S15

After he revealed miniPixs, he realized that he really liked the name Pix and decided to take it! Renaming the miniPix7 that he still had left to MiniPixi.

After S15 n then dr, after his awakening