Homeopathic remedies contain clusters in ultra high diluted solutions above Avogadro number - this is the long awaited proof that homeopathic remedias are not placebo but active remedies! (The term Avogadro limit expresses the fact that, above a certain dilution, mathematically no particles of the original substance are left.) Ultra-high dilutions analysis: Exploring the effects of potentization by electron microscopy, Raman spectroscopy and deep learning --> download scientific study For the past eight years, the dedicated Research Team of the International Academy of Classical Homeopathy, under the guidance of Professor George Vithoulkas and the thorough work of the Head of the Team, Dr Camelia Grosan, has been diligently working to provide conclusive evidence refuting the long-standing skepticism surrounding homeopathic remedies.Through a meticulous combination of advanced analytical techniques including Transmission Electron Microscopy (TEM), Raman Spectroscopy, and Deep Learning (DL) algorithms, this study successfully confirms that homeopathic remedies are active. The investigation focused on evaluating the morphological characteristics of highly diluted solutions of gold (Aurum Met) prepared in both ethanol and water bases across three different levels of potentization (6C, 30C, and 200C). The findings presented in this research definitively settle the debate on the high potencies in homeopathy.The analytical method employed in this study, we believe, will open new vistas in medicine.You will find the paper here: https://www.sciencedirect.com/science/article/pii/S0167732224005932?viaihub The subject of ultrahigh dilutions has been under continuous debate over the years, mainly because the possi bilities to analyze such diluted solutions are limited. In this context, transmission electron microscopy (TEM) investigations were conducted to evaluate the morphological characteristics of ethanol- and water-based highly diluted solutions of gold; three levels of potentization (6C, 30C, and 200C) were examined for each type of solution. Moreover, Raman spectroscopy and deep learning (DL) algorithms were employed for the analysis of the three potentization levels of purified water, unpurified water, and purified water-based gold solutions. Three batches were assessed for each considered category, and the ability to discriminate between all investigated classes, between the potencies within each group or between the classes within the same level of potentization were presented and discussed in correlation with the TEM findings. Distinct forms of organization were revealed by TEM for the three levels of potentization, while the gated recurrent unit (GRU) model showed great accuracy (88 %) for discriminating all classes, over 90 % accuracy for distinguishing the samples within each group and over 95 % accuracy for classification within the same level of potentization if unpretreated Raman spectra were used. Thus, this suite of methods (TEM-EDX and Raman spectroscopy in combination with DL) can be suc cessfully used for the characterization and differentiation of highly diluted solutions resulting after potentization treatment. Furthermore, considering the results obtained from the discrimination study involving all 11 classes and a data augmentation approach, the spectral segmentation method can be seen as a valuable strategy for increasing the model prediction accuracy.All these observations suggest a different organization for the 6C and 30C potencies of AUR and indicate the presence of both nanoparticles and cluster assemblies in the 30C samples. Thus, the clear nanoparticle shape of impurities and the filiform/cluster assemblies formed from small structures promote the idea that at least for AUR 30C, the observed organization is not primarily related to the nanoparticulate systems, but more to large assemblies (clusters) of small structures. Most likely, these clusters also contain ethanol and water (in E-AUR) or water (in W-AUR) molecules, while impurities, either isolated or linked to these large as semblies, appear as nanoparticles (clear round shape and different sizes). Although gold was identified in all the investigated samples, it appears scattered on the grid surface in the TEM-EDX mapping images. The results obtained for the AUR 200C samples suggest an extended organization of AUR 200C molecules, indicating the existence of stable and organized structures over a larger area. The impurities are present as large assemblies (Fig. S10B); thus, large impurities (micrometer scale), such Si, Al, Fe and O, linked together can be seen in the TEM-EDX mapping results. The filiform profile can be also noted for the W-AUR 200C samples (Fig. 3A,3D) as well as the occurrence of some carbon (C)- based structures in E-AUR 200C (Figs. S10A and S10C), and the presence of gold in the two types of investigated 200C samples (Fig. 3 inset and Figs. S9 and S11B). Some of these results are supported by the literature; for example, the presence of gold, even at high potencies (30C and 200C) of AUR that are beyond the Avogadro number, was also shown in the papers of Chik ramane et al. and Rajendran . In the work of Chikramane et al., the elemental composition of TEM particles was identified by selected area electron diffraction (SAED) and confirmed by ICP–AES analysis . The study of Rajendran highlights the idea that nanoparticles are found mostly on the quantum dot scale in all solutions ; their par ticle sizes are approximately similar to our data. This nanoparticulate perspective is also supported by our findings, but in addition, our results demonstrate that AUR’s structure combines nanoparticles and cluster arrangements composed of smaller or larger nanostructures. These cluster arrangements are more evident for the high dilutions that are beyond the Avogadro number and are influenced by the solvent nature and by the level of potentization; the higher the potency is, the more branched and larger the formed structures are. Extension of this orga nization over a large area should lead to structures that are more stable and could be in accord with the constructal law of design and evolution in nature, as described by Bejan and Lorente . Conclusion Overall, by analyzing the results obtained from TEM-EDX and the methodology based on Raman spectroscopy and DL algorithms, we can argue that these methods can be successfully used for the character ization of ultrahigh dilutions. This suite of techniques revealed a modality that could be the basis for discriminating different remedies in accordance with the nature of the initial substances used to prepare the remedies or could allow the differentiation of samples based on their level of potentization. This approach allows the analysis of the highly diluted solutions on a far larger scale than was achievable in the past.--> download scientific study--> IACH international Academy of classical homeopathy in Alonnisos, GreeceTo be successful with homeopathic treatment, you need a highly trained homeopath: --> Homeopaths trained in the International Academy IACH--> elearning teaching at IACH Read the full article
