#b352

1) No smoking, eating, or drinking in laboratory.

2) Shoes or sandals must be worn in laboratory.

3) Familiarize yourself with local poisonous (causing dermatitis) plants, such as poison ivy. *Note: Identify the poison ivy in a group of look alikes

4) Living cultures of Histoplasma capsulatum or Coccidioides immitis are prohibited in laboratory*Note: These are too dangerous for lab *Note: We don't do soil isolation in lab because of Histoplasm capsulatum

5) Label all plates, tubes, slides, etc.*Note: name, date, plate type, medium, etc.

6) Store all slides and cover slips in 4% formaldehyde jars for 24 hours after use if they contain living material *Note: formaldehyde is not dangerous, you can just wash it off.

7) All cultures and contaminated material (e.g., dung) must be autoclaved before discarding. The AI will do this for you. *Note: Autoclaving --> pressure

8) Report all accidents to instructor.

9) Keep fingers out of eyes.

10) Your desk surface will be washed with lysol (working solution) or some other disinfectant after each laboratory session. Do not let this stain sweaty shirts, etc. *Note: we will use quaternary amines (hospital cleaner)

11) If you are a diabetic, routinely use broad-spectrum antibiotics or corticosteroids, are immunosuppressed, or are hypersensitive to poison ivy, please see instructor.

12) Know location of fire extinguisher and how to use it. *Note: Where is it? By an exit.

13) Be careful with long hair over open flames.

14) For spore spills - use formaldehyde jar at once.

15) Phenol (as in lactophenol) is poisonous, corrosive, and rapidly absorbed through the skin and lungs. Do not get KOH or HCl solutions on skin. *Note: just wash it off.

16) No mouth pipetting. Never lick labels.

17) Collecting on campus: be careful, discrete, and conservationally conservative. *Note: no trespassing

18) Watch out for Neurospora, Rhizopus, Aspergillus fumigatus, and A. niger over-growth in dishes.

19) Place coats, purses, book bags, and packages in locations provided, not on desk top.

20) Leave reagent trays and burners on desk top after use.

21) Only B351 or B352 students may ride I.U. vehicles on field trips.

22) Never throw glass, razor blades, or anything else that's sharp into regular trash can; use special disposal container. It is marked "Glass Only." *Note: Plastic pipettes go in this container too

23) It costs more than $220 of taxpayers' money to replace the field diaphragm on out Leitz microscopes when someone forces a diaphragm wide open.

24) DON'T get KOH on microscope objectives; it can "etch" them.