SEKBIO

Diagnostic antigens/antibodies/Kits manufacturer | Specialized in immunodiagnostic We own a veteran team with 30+ years of experience in the IVD industry, experienced in recombinant protein development and large-scale protein manufacturing with comprehensive QC platforms. Provide custom services with powerful IVD assay development capability. To combat the COVID-19 pandemic, our R&D team worked around the clock to develop the COVID-19 antigen/antibody successfully 30 days after the virus broke out in China. With profuse industry resources in IVD, we can assist your demands from product R&D, manufacturing, to registration. Sekbio IVD Services CLIA Reagents Chemiluminescence immunoassay (CLIA) is the most advanced technology developed after radioimmunoassay, enzyme immunoassay, and fluorescence immunoassay. Chemiluminescent immunoassay has the advantages of high sensitivity, strong specificity, wide detection range, simple operation, and a high degree of automation. Covid-19 LFA Uncut Sheet Well-proven with high sensitivity | Massive supply to clients each month | Competitive price Custom Services CLIA reagent development | lateral flow test strip development | Protein/Antibody conjugation service | CRO Service | Custom Antibody Production Service | Recombinant Antibody Production.

Vitamin D is converted to 25-hydroxyvitamin D (25-OH-VD) by hydroxylation in the liver and the active 1, 25-dihydroxy vitamin D in the kidneys. 25 hydroxyvitamin D is the main form of vitamin D in the body. The level of serum 25-OH-VD can reflect the level of vitamin D storage and correlate with the clinical symptoms of vitamin D deficiency. Similar to other steroid hormones, 1, 25-dihydroxy vitamin D3 regulates tissue cell differentiation and related gene expression, mainly regulating blood calcium level and affecting cell differentiation. 25-OH-VD has a high content in blood and a long half-life. It is stable and is not directly affected by diet and lifestyle. Therefore, clinical measurement of 25-OH-VD can reflect the proper level of vitamin D in vivo. 25-OH Vitamin D Intro 25-hydroxyvitamin D is the main form of vitamin D in the body. Vitamin D is a steroid derivative, is a fat-soluble vitamin, and is a cyclopentane poly hydro phenanthrene compound. Vitamin D is mainly synthesized by human skin after being irradiated by ultraviolet rays, and a small part is ingested from food or supplements. Vitamin D affects calcium and phosphorus metabolism and has a wide range of physiological effects. It is an indispensable substance for maintaining human health, cell growth, and development and is closely related to various diseases. There are two forms of vitamin D in the human body, vitamin D3 (cholecalciferol) and vitamin D2 (ergocalciferol), which are converted to 25-hydroxyvitamin D (25-OH-VD) by hydroxylation in the liver, which is then converted to active 1,25-dihydroxy vitamin D in the kidneys. The serum 25-OH-VD can reflect human vitamin D storage level, which is related to the clinical symptoms of vitamin D deficiency. Vitamin D Antibody Vitamin D is an important hormone that regulates calcium and phosphorus metabolism in the body. The skin mainly synthesizes it under sunlight conditions, and a small amount can obtain through diet. The active form of vitamin D is especially 1,25 dihydroxy vitamin D3 (1,25(OH) 2 D 3 ), which exerts physiological effects by binding to the vitamin D receptor (VDR). 25-hydroxyvitamin D (25(OH)D) is the body's primary storage form of vitamin D. 1,25(OH)D3 induces the differentiation of monocytes into macrophages and reduces the release of inflammatory cells and chemokines.

A tumour marker is a chemical substance that reflects the presence of a Tumor. It is present in malignant tumour cells, not in normal adult tissues, and substances abnormally produced by malignant tumour cells or by the host's response to tumour stimulation created it. Their presence or quantitative changes can indicate the nature of the tumour to understand the histogenesis, cell differentiation, and cell function of the tumour. Detection of tumour markers has the following functions: Early detection of tumour; Tumour census and screening; Diagnosis, differential diagnosis and staging of tumours; Monitoring the efficacy of surgery, chemotherapy and radiotherapy for tumour patients; Indicators of tumour recurrence; Prognosis of tumour; Looking for the primary foci of metastases of unknown origin. Tumor Antigens Tumor antigens generally refer to new or overexpressed antigenic substances during the occurrence and development of tumors. The possible mechanisms for the body to produce tumor antigens are: 1) gene mutation; 2) the activation of genes that were not expressed initially during the process of cell carcinogenesis; 3) abnormalities in some aspects of the antigen synthesis process (such as abnormal glycosylation leading to the production of unique protein degradation products) ); abnormal and ectopic expression of antigens or differentiation antigens during embryonic stage; ⑤ overexpression of specific gene products, significantly signal transduction molecules; ⑥ expression of exogenous genes (such as viral genes). Cancer Antigen According to the specificity of cancer antigens, cancer antigens are divided into (1) Tumor-specific antigens (2) Tumor-associated antigens. Tumor-specific antigen (TSA) is a neoantigen-specific to tumor cells or exists only in specific but not in normal cells. Tumor-associated antigen (TAA) refers to antigens peculiar to non-tumor cells and exist in normal cells and other tissues. Still, their content is significantly increased when cells become cancerous.

Triiodothyronine, also known as T3, is a thyroid hormone. It affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate. The determination of triiodothyronine is one of the sensitive indexes in the diagnosis of hyperthyroidism. When elevating hyperthyroidism, it is also the precursor of the recurrence of hyperthyroidism. In addition, pregnancy and acute hepatitis will advance Hypothyroidism, simple goiter, acute and chronic nephritis, chronic hepatitis, cirrhosis of the liver. T3 can be used to diagnose T3-hyperthyroidism, early detection of hyperthyroidism, and diagnosis of pseudo thyrotoxicosis. The serum T3 and T4 levels changes are generally consistent, which is a sensitive indicator of thyroid function diagnosis, especially important for early diagnosis. It is a specific diagnostic indicator of T3 hyperthyroidism. Triiodothyronine Intro As the largest endocrine gland in the human body, the thyroid secretes the main active substances, tetraiodothyronine (T4) and triiodothyronine (T3), which are extremely important for protein synthesis thermoregulation, energy production, and regulation effect. T3 plays a vital role in judging the physiological condition of the human body, so it is of great significance to detect the content of T3 in serum. The content of T3 in serum is 1/80~1/50 of that of T4, but the biological activity of T3 is 5~10 times that of T4. Most of the T3 in serum is converted from the deiodination of peripheral tissues. A small part of T3 is directly secreted by the thyroid and released into the blood. Most of the T3 in serum is bound to binding proteins. About 90% is bound to thyroxine-binding globulin (TBG), the rest is bound to albumin, and a tiny amount is attached to thyroxine-binding prealbumin (TBPA). Free T3 Test Increased T3 is seen in hyperthyroidism, early stage of triiodothyronine-type hyperthyroidism crisis, iodine-deficiency goiter, and hyperthyroid-binding globulinemia. It is decreased in hypothyroidism, low thyroxine-binding globulinemia, and so on. High result possible disease: hyperthyroidism. Low result possible diseases: Hypothyroidism, endocrine proptosis, thyroiditis, resting lymphocytic thyroiditis, hyperthyroidism in children, hyperthyroidism in the elderly, thyroid tuberculosis, endocrine disorders, euthyroid goiter, precocious children Breast hypertrophy.

The TSH receptor is a member of the G protein-coupled receptor superfamily of integral membrane proteins. The thyrotropin receptor (or TSH receptor) locates primarily in the body of the thyroid gland. It recognizes and binds to stimulating thyroid hormone (also known as "thyrotropin") secreted from the pituitary gland. TSH stimulates the production of thyroxine (T4) and triiodothyronine (T3). TSHR is closely related to the pathogenesis of various autoimmune thyroid diseases and is an essential indicator for the clinical diagnosis of these diseases. SEKBIO is now offering Mouse anti-human TSHR mAb, Humanized anti-human TSHR mAb, Recombinant human sTSHR (Soluble), and human TSHR extractive of cell membranes (Full length) for diagnosis usage TSHR Thyroid-stimulating hormone receptor (TSHR) and its homologous luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor belong to a class of glycoprotein receptors. They are also visual A member of the large family of G protein-coupled receptors (GPcRs) of rhodopsin (approximately 45% identity at the amino acid level). TSHR is mainly expressed in thyroid follicular cells and activates the protein kinase A (PKA) and phospholipase C (Phospholipases C, PLc) signal transduction systems after combining with thyroid-stimulating hormone (TSH) under physiological conditions. , regulate the growth and differentiation of the thyroid, and promote the synthesis and release of thyroid hormones. Anti TSH Antibody The expression of TSHR can also be found in other cells or tissues, including adipocytes, fibroblasts, osteoblasts and osteoclasts, bone marrow cells and cardiomyocytes, etc., and is expressed in embryonic stem cells at an early stage. In autoimmune thyroid diseases, especially toxic diffuse goiter (Graves' disease, GD), TSHR can act as an autoantigen and bind to thyroid-stimulating antibody (TSAb) to produce TSH-like stimulation. The reaction will eventually lead to the loss of thyroid autoimmune tolerance, production and release of excessive thyroid hormones, and clinical manifestations of hyperthyroidism such as palpitations, sweating, and hyperthyroidism. Therefore, studying the structure of thyroid-stimulating hormone receptors and elucidating the interaction between autoantibodies and thyroid-stimulating hormone receptor (TSHR) molecules is vital for understanding Graves disease's pathogenesis, a common organ-specific autoimmune disease, and guiding clinical practice. Diagnosis and treatment have far-reaching significance.

Thyroid peroxidase (TPO), also called thyroperoxidase or iodide peroxidase, catalyzes the iodization of thyroglobulin tyrosine during the biosynthesis of T3 and T4. TPO is an enzyme expressed mainly in the thyroid, where it is secret into the colloid. The increased TPO level may reflect the promoted thyroid hormone synthesis, which may cause hyperthyroidism. The detection of TPO helps with diagnosing Hashimoto's disease (HD) and autoimmune hyperthyroidism; Toxic chronic goiter (Graves); Monitoring the effect of immunotherapy; To detect the possibility of familial thyroid disease; Prediction of postpartum thyroid dysfunction in pregnant women. SEKBIO provides Humanised anti-human TPO mAb for diagnosis usage. Thyroid Peroxidase Intro Thyroid peroxidase(TPO) TPO is an important enzyme that catalyzes thyroid hormones. TPO is synthesized by thyroid follicular cells. It is a 10% glycated hemoglobin-like protein composed of 933 amino acid residues with a molecular weight of 103 kD. It is most abundantly distributed in the microvilli on the follicular lumen surface. TPO uses hydrogen peroxide as an oxidant. TSH regulates the generation and activity of TPO. In the experiment, the action of TPO disappeared 48 hours after removing the rat pituitary, and TPO activity will restore after the injection of thyroid-stimulating hormone. Thiourea drugs can inhibit the activity of TPO, thus inhibiting the synthesis of thyroid hormones, and are commonly used clinically for the treatment of hyperthyroidism (hyperthyroidism). Thyroid Peroxidase Antibody Thyroid peroxidase (TPO) is a membrane-bound heme protein with a molecular weight of about 100,000. TPO participates in two different reactions in the biosynthesis of thyroid hormones. If thyroid peroxidase antibodies are present, it is a sign of concomitant damage to thyroid function or potential harm. Peroxidase antibody-positive suggests that the thyroid disease may be Hashimoto's thyroiditis.

The thyroid gland is a 2-inches long, butterfly-shaped endocrine gland generally located in the lower front of the neck. The thyroid functions to make thyroid hormones, which are released into the blood and then carried to every tissue in the body. Thyroid hormones regulate the body using energy, maintaining body temperature, and keeping the brain, heart, muscles, and other organs working. For this reason, an imbalance in thyroid hormone levels can negatively affect your bodily functions and your mood. Thyroid tests are used to diagnose thyroid function by detecting thyroid hormone markers in the blood. The specific blood tests to test your thyroid can include Thyroglobulin (Tg), Thyroid Peroxidase (TPO), Thyroid Stimulating Hormone Receptor (TSHR), Triiodothyronine (T3), etc. Thyroid Function Intro The physiological function of the thyroid is mainly to promote the metabolism of three major nutrients, regulate growth and development, increase tissue oxygen consumption, promote energy metabolism, increase heat production and improve basal metabolism. When thyroid dysfunction occurs, hyperthyroidism or hypothyroidism will occur. The hypothalamus-pituitary-thyroid axis mainly regulates the synthesis and secretion of thyroid hormones. Thyroxine (T4) and triiodothyronine (T3) are synthesized in thyroid follicular epithelial cells. Under the stimulation of pituitary thyroid-stimulating hormone (TSH), they are released into the blood after a series of changes. All processes from iodine uptake to release in thyroxine synthesis have a promoting effect. More than 99% of T3 and T4 are bound to plasma proteins, mainly attached to thyroxine-binding globulin. Only about 0.4% of total plasma T3 and 0.04% of T4 are free, and only free T3 and T4 can Enter the target cells to play a role, and the part bound to the protein regulates and stabilizes free T3 and T4. The level of free T3 and T4 in the blood Fluctuating negative feedback causes an increase or decrease in the release of thyrotropin-releasing hormone (TRH) from the hypothalamus and the release of thyrotropin-releasing hormone (TSH) from the pituitary. The primary role of TRH is to promote the synthesis and release of TSH from the pituitary. Thyroid Antibody (1) Thyroglobulin antibody (TGA) and thyroid microsomal antibody (TMA): TGA and TMA in serum are two primary specific thyroid autoantibodies. TGA is a specific diagnostic indicator for chronic lymphocytic thyroiditis, often significantly elevated. Autoimmune thyroid disease TGA and TMA are elevated and can also be detected in the blood of other thyroid diseases and healthy people. (2)Thyrotropin receptor antibody (TRAb): TRAb is also known as a thyroid-stimulating antibody (TSAb) or thyroid-stimulating immunoglobulin (TSI). TRAb is a kind of thyroid autoantibody produced in the autoimmune process of malignant diffuse goiter. It can stimulate the thyroid to produce thyroid hormones. The determination of TRAb is beneficial to the study of the pathogenesis of diffuse malignant goiter.

Thyroglobulin is a 660 kDa, a dimeric glycoprotein produced by the follicular cells of the thyroid and released into the residual cavity of the thyroid follicles; the entire process happens in the thyroid gland. TSH, iodine deficiency in the thyroid, and thyroid-stimulating immunoglobulin can stimulate Tg. A thyroglobulin test mainly acts as a tumor marker test to help guide thyroid cancer treatment. Tg can distinguish congenital complete thyroid defect and hypothyroidism. Thyroglobulin is also considered a specific marker of thyroid body integrity. It is a tumor marker for differentiated thyroid cancer (DTC) and can use as an essential reference index for follow-up patients with differentiated thyroid cancer after treatment. It can also distinguish subacute thyroiditis from pseudo thyrotoxicosis. Thyroglobulin Intro Thyroglobulin (Tg) is a macromolecular glycoprotein secreted by thyroid follicular epithelial cells, most synthesized by thyroid cells and released into the remnant cavity of thyroid follicles. Factors such as TSH, iodine deficiency in the thyroid, and thyroid-stimulating immunoglobulin can stimulate its production. In patients with congenital hypothyroidism, detection of Tg can differentiate between complete thyroid defect and thyroid hypoplasia. Thyroglobulin is also considered a specific marker of thyroid body shape integrity. It is a tumor marker of differentiated thyroid cancer (DTC) and can be used as an important reference index for follow-up patients with differentiated thyroid cancer after treatment. It can also use to differentiate subacute thyroiditis from pseudothyrotoxicosis. Thyroglobulin Antibody Thyroglobulin antibody is a common autoantibody in the serum of patients with autoimmune thyroid disease, mainly composed of IgG1, IgG2, and IgG4, and a small part of IgA and IgM. After thyroglobulin antibodies bind to thyroglobulin, they can interact with the bound antibodies through Fc receptors, activate NK cells, and attack target cells, destroying thyroid cells. It is generally believed that thyroglobulin antibodies have no damaging effect on the thyroid gland.

Testosterone is the primary male sex hormone and an anabolic steroid. In male humans, testosterone plays a vital role in developing male reproductive tissues such as the testes and prostate and promoting secondary sexual characteristics such as increased muscle and bone mass and the growth of body hair. The increased Testo level may be caused by: tumor, hermaphroditism, polycystic ovary syndrome, etc. The decreased level reflects testis hypoplasia, hypophysis of the anterior lobe, cancer, etc. Combined with serum LH and FSH test results, it can identify primary or secondary testicular dysfunction and precocious puberty in adolescent children. Testosterone Intro Testosterone, with the molecular formula C19H28O2, is a steroid hormone secreted by the testes of men or the ovaries of women. It has the functions of maintaining muscle strength and mass, maintaining bone density and strength, refreshing, and improving physical fitness. The adrenal glands also secrete a small amount of testosterone. Testosterone is a secreted glycoprotein initially identified from the enrichment medium of mouse Sertoli cells. A small molecular weight cytoskeleton-associated protein binds tightly to the cell surface through receptor-associated proteins.