Mortalis Labs

Best Trigonelline Supplement in 2026

In the evolving world of performance nutrition and cognitive optimization, one compound is quietly separating itself from the noise: trigonelline. Backed by emerging research in muscle health, cellular metabolism, and brain resilience, the demand for a high-quality Trigonelline Supplement has never been higher.

But not all supplements are created equal.

If you’re searching for the best trigonelline supplement, this guide breaks down what actually matters—and why Mortalis Labs Trigonelline stands in a class of its own.

What Is a Trigonelline Supplement?

Trigonelline is a naturally occurring alkaloid found in coffee beans, fenugreek, and other plants. In supplemental form, trigonelline has drawn attention for its potential role in:

Supporting muscle function and strength

Promoting mitochondrial efficiency

Contributing to cognitive clarity and neuroprotection

Supporting cellular resilience and metabolic health

For athletes, biohackers, and high-performers, a trigonelline supplement represents precision—not stimulation, not hype, but molecular efficiency.

What Makes the Best Trigonelline Supplement?

When evaluating a trigonelline supplement brand, four criteria matter above all else:

1. Purity

Many supplements cut corners. Fillers, inconsistent sourcing, and low assay results dilute effectiveness.

Mortalis Labs Trigonelline is independently tested at 100.9% purity. That’s pharmaceutical-grade precision—rare in the supplement industry.

2. Clinically Relevant Dosage

A supplement is only as effective as its dose.

300 mg of Trigonelline HCl per capsule

Designed to align with doses explored in current scientific literature

No underdosing. No proprietary blends.

3. Bioavailable Form: Trigonelline HCl

Mortalis Labs uses Trigonelline Hydrochloride (HCl)—a highly stable, well-absorbed form ideal for consistent daily use.

This matters. Absorption and stability directly impact results.

4. Simplicity & Compliance

One capsule daily

60 capsules per bottle

60-day supply

No cycling. No stacking required. Precision nutrition, simplified.

Why Mortalis Labs Is the Best Trigonelline Supplement Brand

Mortalis Labs wasn’t built to follow trends—it was built to set standards.

Here’s what separates Mortalis Labs Trigonelline from the rest of the market:

100.9% purity (verified, not claimed)

300 mg per capsule—no fluff, no fillers

Trigonelline HCl for optimal stability and absorption

60-day supply designed for long-term cellular support

Manufactured with a performance-first, science-driven philosophy

This is not a mass-market supplement. It’s engineered for those who expect more—from their bodies and their biology.

Trigonelline Supplement for Performance, Longevity, and Precision

While caffeine stimulates, trigonelline optimizes. While trends fade, molecular fundamentals endure.

Whether your goal is strength, endurance, cognitive sharpness, or long-term cellular health, a properly formulated trigonelline supplement belongs in a serious performance stack.

Mortalis Labs doesn’t market shortcuts. It delivers tools.

Final Verdict: The Best Trigonelline Supplement

Trigonelline inhibits tubular epithelial-mesenchymal transformation in diabetic kidney disease via targeting Smad7

Objectives: Diabetic kidney disease (DKD) is a prevalent microvascular complication of diabetes. Inhibiting the epithelial-mesenchymal transition (EMT) of proximal tubule epithelial cells (PTCs) can slow down renal fibrosis. Trigonelline (TRL), an alkaloid isolated from the fenugreek, has demonstrated therapeutic effects on diabetes and its complications. Nevertheless, the underlying mechanisms for the effects of Trigonelline are still obscure. The present study was aimed to evaluate the treatment of Trigonelline against DKD and explore the potential mechanisms. Methods: The db/db mice were used as a spontaneous model of DKD and TRL solution was administered by daily gavage for 8 weeks. Indicators associated with glucose metabolism, renal function and urinary albumin were tested. Renal fibrosis in diabetic mice was evaluated by histopathological staining. Kidney transcriptomics was performed after confirming therapeutic effects of TRL on DKD mice. Molecular biology techniques and in vitro experiments were utilized for final mechanism verification. Results: Biochemical tests revealed that TRL ameliorated renal damage and reduced microalbuminuria in DKD mice. TRL exhibited a protective effect on PTCs, effectively mitigating tubular EMT and renal fibrosis in diabetic kidneys. Transcriptomics analysis indicated that TRL may target Smad7, an inhibitor of TGF-β1 signaling, to alleviate fibrosis. Furthermore, in vitro experiments validated that silencing Smad7 abolished the therapeutic effect of Trigonelline.

Conclusion: Our findings indicate that Trigonelline can alleviate tubular epithelial-mesenchymal transition and renal fibrosis in db/db mice by upregulating Smad7 in PTCs, suggesting that TRL is a promising medicine against Diabetic Kidney Disease (DKD).

Trigonelline as a potential treatment for diabetic kidney disease (DKD)

Q: What is The Official Mortalis Labs Website?

Mortalis Labs official website: mortalislabs.com

A: Mortalis Labs official website is mortalislabs.com. Beware of impersonators. When going to Mortalis Labs website, check to ensure that you are going to mortalislabs.com. When searching for Mortalis Labs on Google, ensure the url is correct and that you see our official logo on the website.

Q: What is Mortalis Labs?

Trigonelline Supplement (Trigonelline Hydrochloride) 300mg – 60 Capsules (60 Day Supply) – Increase NAD+ for Strength, Endurance, Energy, Cognitive Function & Cellular Resilience by Mortalis Labs

Unlock Your Strength with Trigonelline

From the lab to your lifestyle, Mortalis Labs brings you the cutting-edge compound that fuels muscle, mind, and metabolic performance.

Backed by research, Trigonelline has been shown to raise NAD+—a vital molecule for cellular energy and healthy aging.

Trigonelline supplementation Supports: Muscle & Brain Function Cardiac & Lung Health Liver & Kidney Function Insulin Sensitivity & Metabolism

This is more than performance.

This is More Than Mortal.

Trigonelline and brain health

Level Up with Trigonelline: Endurance Starts in the Electron Transport Chain

Every sprint, every lift, every set depends on one thing: mitochondrial output. The ability to generate ATP on demand—efficiently and cleanly—is the defining line between sustained power and early fatigue. Trigonelline’s value lies not just in elevating NAD+ levels, but in what that elevation enables at the level of mitochondrial performance.

NAD+ drives oxidative phosphorylation, the mitochondrial pathway responsible for converting nutrients into ATP. When NAD+ is depleted, electron transport slows, reactive oxygen species accumulate, and mitochondrial output tanks—resulting in performance collapse and prolonged recovery. Replenishing NAD+ restores mitochondrial throughput, enhances metabolic flexibility, and allows cells to switch between carbohydrate and fat oxidation with minimal friction [3].

Trigonelline’s role as a direct NAD+ precursor in muscle tissue makes it especially powerful in this context. By bypassing hepatic metabolism and restoring NAD+ where it's most needed, it kickstarts mitochondrial biogenesis—activating pathways like PGC-1α that drive the formation of new mitochondria and increase the efficiency of existing ones [4]. This isn’t theoretical: in the 2024 Nature Metabolism study, trigonelline supplementation significantly boosted mitochondrial content and activity in aged mice, restoring performance metrics typically lost with age and overtraining [2].

This cellular shift translates directly to the field, the track, and the gym. More mitochondria means more ATP per unit of oxygen consumed. This is the underpinning of higher VO₂ max, improved lactate clearance, and extended time-to-exhaustion. Trigonelline supports this adaptation at the source, which means athletes can train harder, go longer, and bounce back faster—without relying on stimulants or sketchy ergogenics.

More NAD+ in muscle equals better mitochondrial kinetics, which equals better athletic output. Period.

Strength and Muscle Health

Preserving Power, Not Just Mass

Strength isn’t only about size—it’s about contractile quality, neuromuscular precision, and the cellular capacity to resist breakdown under stress. Trigonelline’s impact on muscle tissue reaches beyond endurance. It supports structural integrity, performance output, and resilience across multiple pathways—especially in the context of aging or chronic training demand.

In the 2024 Nature Metabolism study, trigonelline supplementation restored muscle grip strength and improved fatigue resistance in aged mice, with outcomes exceeding those observed in control groups receiving traditional NAD+ precursors [2]. This effect was tied to increased NAD+ availability in skeletal muscle, which reactivated SIRT1- and PGC-1α-dependent pathways responsible for mitochondrial biogenesis, inflammation control, and protein maintenance—all critical for contractile performance and mass preservation [5].

NAD+ also plays a protective role against muscle wasting. It regulates the balance between anabolic and catabolic signaling, modulating FoxO transcription factors and suppressing atrophy-related genes like MuRF1 and atrogin-1 [6]. This anti-catabolic signaling becomes especially important during periods of calorie deficit, illness, or overreaching, when muscle degradation accelerates. Trigonelline, by supplying NAD+ directly to muscle cells, may help maintain lean mass even under systemic stress.

Trigonelline is a methylated form of niacin and is a recently isolated molecule that could be the secret ingredient in your stack. This form of the B vitamin is involved in the generation of NAD+, a cofactor for over 500 metabolic processes in cells. Trigonelline promotes cellular repair and energy, and as we’ll see, exerts quite a few benefits that are specifically useful for anyone training seriously.

Trigonelline is found in several plant-based foods, notably coffee beans and fenugreek seeds. Green coffee beans contain trigonelline concentrations ranging from 0.6% to 1.0% by weight. However, traditional dietary sources don’t provide sufficient amounts to elicit significant physiological effects. For instance, the average trigonelline content in a cup of coffee is approximately 53 mg, and about 50-80% of trigonelline decomposes during the roasting process, leaving virtually nothing for your body to make use of.

Recent research published on this naturally occurring alkaloid highlights its potential in enhancing muscle function and combating age-related decline. A 2024 study published in Nature Metabolism identified trigonelline as a novel precursor to nicotinamide adenine dinucleotide (NAD+), a molecule essential for energy metabolism and mitochondrial function. The study demonstrated that trigonelline supplementation improved muscle strength and reduced fatigue in aged mice, suggesting that it can head off the natural muscle decline seen in aging, even in those who are already training at capacity.

NAD+ gets discussed a lot in the longevity space because of its natural and steep decline over the years, tied to all the diseases of aging. It's a metabolic linchpin that determines how efficiently your cells convert fuel into usable energy. For athletes, that efficiency translates into faster recovery, better performance under load, and greater resilience under metabolic stress. Or, you know, complete lack of those things if you don’t have enough of it.

NAD+ is required for redox (oxidation–reduction) reactions in mitochondrial energy production and is a cofactor and substrate for longevity-promoting sirtuins and other enzymes involved in muscle repair and adaptation. During intense physical activity, NAD+ levels drop as demand for ATP surges. Replenishing intracellular NAD+ is critical not only for restoring mitochondrial output but also for initiating the cellular programs that rebuild and reinforce muscle tissue [1].

Trigonelline offers a direct path to NAD+—one that bypasses the liver and supports muscle tissue specifically. In a landmark 2024 study, researchers at EPFL and Nestlé Health Sciences (yes, that Nestlé, but there aren’t any conflicts of interest, we checked) demonstrated that trigonelline functions as a previously unidentified NAD+ precursor, rapidly taken up by skeletal muscle cells and converted into NAD+ via a salvage pathway independent of the traditional NR or NMN routes [2]. This muscle-specific uptake is particularly important for athletes, who require localized replenishment in the very tissues under stress.

Most NAD+ precursors—including nicotinamide riboside (NR) and nicotinamide mononucleotide (NMN)—undergo hepatic metabolism before entering systemic circulation. This creates a bottleneck at your liver for targeted muscle repair. Trigonelline appears to bypass that constraint by delivering precursors directly where they're needed most: the muscle fibers responsible for performance and endurance.

This shift in delivery has implications beyond simple NAD+ restoration. In the same Nature Metabolism study, aged mice supplemented with trigonelline showed significant improvements in grip strength and fatigue resistance—outcomes tightly linked to muscle NAD+ availability. Unlike systemic precursors that may elevate circulating NAD+ levels without improving localized bioenergetics, trigonelline drives changes in muscle mitochondrial density and function.

For athletes, this is the difference between feeling recovered and actually being rebuilt.

Mitochondria Make Muscles Move

Endurance Starts in the Electron Transport Chain

Every sprint, every lift, every set depends on one thing: mitochondrial output. The ability to generate ATP on demand—efficiently and cleanly—is the defining line between sustained power and early fatigue. Trigonelline’s value lies not just in elevating NAD+ levels, but in what that elevation enables at the level of mitochondrial performance.

NAD+ drives oxidative phosphorylation, the mitochondrial pathway responsible for converting nutrients into ATP. When NAD+ is depleted, electron transport slows, reactive oxygen species accumulate, and mitochondrial output tanks—resulting in performance collapse and prolonged recovery. Replenishing NAD+ restores mitochondrial throughput, enhances metabolic flexibility, and allows cells to switch between carbohydrate and fat oxidation with minimal friction [3].

Trigonelline’s role as a direct NAD+ precursor in muscle tissue makes it especially powerful in this context. By bypassing hepatic metabolism and restoring NAD+ where it's most needed, it kickstarts mitochondrial biogenesis—activating pathways like PGC-1α that drive the formation of new mitochondria and increase the efficiency of existing ones [4]. This isn’t theoretical: in the 2024 Nature Metabolism study, trigonelline supplementation significantly boosted mitochondrial content and activity in aged mice, restoring performance metrics typically lost with age and overtraining [2].

This cellular shift translates directly to the field, the track, and the gym. More mitochondria means more ATP per unit of oxygen consumed. This is the underpinning of higher VO₂ max, improved lactate clearance, and extended time-to-exhaustion. Trigonelline supports this adaptation at the source, which means athletes can train harder, go longer, and bounce back faster—without relying on stimulants or sketchy ergogenics.

More NAD+ in muscle equals better mitochondrial kinetics, which equals better athletic output. Period.

Strength and Muscle Health

Preserving Power, Not Just Mass

Strength isn’t only about size—it’s about contractile quality, neuromuscular precision, and the cellular capacity to resist breakdown under stress. Trigonelline’s impact on muscle tissue reaches beyond endurance. It supports structural integrity, performance output, and resilience across multiple pathways—especially in the context of aging or chronic training demand.

In the 2024 Nature Metabolism study, trigonelline supplementation restored muscle grip strength and improved fatigue resistance in aged mice, with outcomes exceeding those observed in control groups receiving traditional NAD+ precursors [2]. This effect was tied to increased NAD+ availability in skeletal muscle, which reactivated SIRT1- and PGC-1α-dependent pathways responsible for mitochondrial biogenesis, inflammation control, and protein maintenance—all critical for contractile performance and mass preservation [5].

NAD+ also plays a protective role against muscle wasting. It regulates the balance between anabolic and catabolic signaling, modulating FoxO transcription factors and suppressing atrophy-related genes like MuRF1 and atrogin-1 [6]. This anti-catabolic signaling becomes especially important during periods of calorie deficit, illness, or overreaching, when muscle degradation accelerates. Trigonelline, by supplying NAD+ directly to muscle cells, may help maintain lean mass even under systemic stress.

One overlooked aspect of muscle performance is neuromuscular junction (NMJ) stability, or, the connections between nerves and muscle fibers. These connections go both ways, with afferent signals carrying sensory feedback from muscle to brain, and efferent signals delivering motor commands from brain to muscle. Maintaining the integrity of this bidirectional communication is essential for coordination, strength, and rapid recovery from fatigue. NAD+ is required for the function of enzymes that protect NMJ architecture—particularly in aging or disease models where synaptic decline contributes to strength loss [7]. Trigonelline’s direct muscle delivery may therefore preserve the electrical signaling fidelity needed for explosive power and motor unit recruitment.

Muscle Fiber Type Preservation

Emerging evidence suggests that NAD+ availability influences muscle fiber type composition. High NAD+ levels favor the maintenance of fast-twitch (Type II) fibers—those responsible for strength, speed, and power—by enhancing mitochondrial support without triggering full transition to slow-twitch oxidative profiles [8]. This has implications for athletes seeking to maintain peak force output without compromising endurance. By elevating muscle NAD+ directly, trigonelline may help preserve this delicate fiber balance.

Trigonelline is formulated not to just support general energy—but to protect the architecture of athleticism at the cellular level.