Dr. Ahmed Majeed Al-Shammari

Objective: This study aimed to the preparation of stable colloidal silver nanoparticles (AgNPs) and test the novel combination therapy, which comprises AgNPs and lives attenuated measles virus (MV) vaccine, to target breast cancer cells. the safety of the proposed therapy in normal breast epithelial cell lines (HBL-100) was tested. Methods: Silver nanoparticles were prepared by chemical reduction, The stability, size, and concentration of the colloidal component have been demonstrated by examining the ultraviolet-visible (UV-Vis) spectroscopy at a different time and Zeta potential examination, atomic force microscopy (AFM), and atomic spectroscopy. MV was propagated in the African green monkey kidney cell line (VERO-hSLAM cells). Cytotoxicity assay was evaluated on Michigan Cancer Foundation-7 (MCF-7) and human breast cancer cell line (CAL-51). The safety of the proposed therapy in HBL-100 was tested. Results: The formation of nanoparticles is confirmed by the appearance of a perfect surface plasmon resonance (SPR) band at 424 nm. The stability was proved by a slight change in absorption intensity from 224 nm to 226 nm (immediately and after a month) respectively, and the value of the charge which was -41.13 mV. NPs were spherical in shape and an average diameter of 40.87 nm. The concentration was 13 µg/ml. The Chou–Talalay analysis revealed synergism between the MV and AgNPs in all tested cancer cell lines while Normal epithelial HBL-100 cells well tolerated all treatment modalities. Conclusion: The novel combination showed effective antitumor activity against breast cancer cells and high safety in normal cells.

β-TCP-coated (Ti and zirconia) implants generated periodontal tissue and formed biohybrid implants when mesenchymal-tissue-layered cell sheets were isolated from PDLSCs alone or co-cultured BMMSCs and PDLSCs.

A characteristic of cancer cells is increased glucose uptake and glycolysis for energy production and hydroperoxide detoxification due to mitochondrial dysfunction. Thus, inhibition of glucose uptake and glycolysis represent smart novel therapy. We used 2-deoxyglucose (2DG) as a glycolysis inhibitor and acarbose (ACA), a specific alpha-glucosidase inhibitor, to decrease glucose uptake. Mice bearing mammary adenocarcinoma tumors were treated by 2DG and/or ACA. Relative tumor volume, tumor growth inhibition rate, relative body weight, glucose concentration, hexokinase-1 protein level by ELISA, pyruvate, and ATP (glycolysis products), reactive oxygen species (ROS), total glutathione T-GSH, apoptosis, and histopathology were measured in treated and untreated groups. Our results showed that combination therapy inhibited tumor volume and increased tumor growth inhibition rate, body weight reduction, decreasing glucose level, HK-1 level, and inhibition of glycolysis products. In addition, combination therapy induced oxidative stress, increase ROS, and decrease T-GSH. Furthermore, immunohistochemistry examination showed the broader area of apoptosis in breast cancer treated by combination agents. In conclusion, our result revealed that the novel combination inhibits glycolysis and glucose uptake and induced oxidative stress and apoptosis.

Direct Reprogramming of Mice Skin Fibroblasts into Insulin-Producing Cells In Vitro

25 posts!

My new established cancer cell line

6:30 PM - 6:45 PM 12: Virulent Velogenic Newcastle Disease Virus is More Oncolytic Than Attenuated and Lentogenic Newcastle Viruses Ahmed Majeed Al-Shammari, Ph.D., Mustansiriyah University, Iraqi Center for Cancer and Medical Genetic Research

In this Research Topic, we are looking for improving of cancer therapeutics through spotting the light on the novel, innovative manuscripts about recent and promising research on oncolytic virotherapy and ways to enhance its efficacy against cancer and related applications. We welcome preclinical in vitro and in vivo research work, translational research, and clinical studies. We welcome the submissions of original research and review articles covering, but not limited to, the following sub-topics: • Viro-immunotherapy • Combination therapeutics • Nano-virotherapy • Armed therapeutic viruses • Reprogrammed viruses for cancer targeting • Tumor imaging using oncolytic viruses • Cancer-targeting gene-viro-therapy • Modeling the efficacy of oncolytic viruses in vitro and in vivo

منذ سبع سنوات ومستمرون

ومستمرون بتطوير العلاج

ASGCT 

Investigating the anti-proliferation activity of Conocarpus erectus Against breast tumor cells in vitro

تهدف الورشة الى التعريف بماهية العلاج الفايروسي وكيفية استهداف مسار ايض الكلوكوز في الخلايا السرطانية كهدف علاجي مهم حيث تعتمد الخلايا السرطانيية على مسار الايض الهوائي للكلوكوز فقط لانتاج الطاقه وبالتالي فان استهداف هذا المسار وتثبيطه سيكون هدفا مهما لتجويع الخلايا السرطانية وحماية الخلايا الطبيعيه التي تمتلك مسار اخر يمكنها من انتاج الطاقه. اوضحت التجارب البحثية ان تثبيط مسار ايض الكلوكوز باستخدام مثبطات متخصصه وتعريض الخلايا السرطانية في نفس الوقت للعلاج الفايروسي اضهرت فعلا مضادا للاورام بشكل اكبر واقوى من العلاج المنفرد. Most cancer cells exhibit increased glycolysis and use this metabolic pathway cell growth and proliferation. Targeting cancer cells’ metabolism is a promising strategy in inhibiting cancer cell progression. We used specific hexokinase inhibitors, to inhibit glycolysis to enhance the Newcastle disease virus anti-tumor effect. this presentation to explain this novel anti-tumor new strategy. The slides in English but the talk is in Arabic.

An Arabic explanation to the oncolytic virotherapy 

Novel finding to my team, we can achieve great tumor growth inhibition using this novel combination of NDV and glycolysis inhibitor