#transfectiontechniques

Transfection is the process of introducing nucleic acids, either naked or purified, into eukaryotic cells. As we know, a cell where the nucleus is enclosed within a nuclear membrane is called a eukaryotic cell. Large and complex organisms like plants and animals have eukaryotic cells. What are nucleic acids? Well, DNA and RNA. And what is the use of introducing nucleic acids into eukaryotic cells directly? Such a process is undertaken to essentially change the core properties of the cell itself.  Here are some of the tips to carry out a successful transfection.

One of the powerful tools to study gene regulation and protein function is transfection. Transfection is a process of introducing nucleic acids like DNA, RNA, siRNA, and oligonucleotides into cells without the use of viral infection. Transfection contributes to the development and advancement of cellular research and drug discovery. The two significant purposes of transfection are - a) to enhance gene expression, b) to produce recombinant proteins. 

Transfection is cost-effective and produces recombinant human therapeutic proteins in ample amount, which used to be one of the obstacles in biopharmaceutical industries. Transient cell transfection pulled off these results by avoiding a lengthy and expensive procedure of developing stable transfectants. Transfection has also been used to producer-proteins, viral vectors for therapeutic and gene therapy.

Transfection is used by researchers to study genetics as well as cellular and molecular biology. To produce results that are accurate and reproducible, therefore, the underlying mechanisms of transfection must be understood, as well as the different parameters that might affect it. Listed below are seven important tips meant to help researchers get more reliable results through transfection.

MarinBio collaboration revolves around stable transfection when clients need a Custom Assay Development for long-term drug release. We have expertise in gene expression, DNA RNA drugs, protein production, stable cell lines.

Transfection refers to the introduction of nucleic acid straight into eukaryotic cells by implementing non-viral methods. Essentially, it’s a gene transfer process that makes use of various physical and chemical methods to facilitate the study of protein as well as gene function within a cellular environment. When looking to rely on laboratory-based tests for getting transfection services, few things play quite a significant role. Among all others, the important ones include Cell Health, DNA quality, confluency, serum, antibiotics, and others. In this post, we take a closer look at discussing the factors influencing transfection services that contribute towards establishing efficiency. However, it is to be noted that with transfection methods and reagents, a certain degree of cell death is inevitable. Read on to gain an effective insight into the factors involved.

We currently have various chemical, biological, and physical methods to deliver nucleic acids to the cell cultures. However, not every method is suitable for every experiment or cell culture. In fact, the transfection efficiency, normal physiology effects, cell toxicity, and gene expression widely vary in different methods, which are used for different cell cultures. It is still possible to broadly define all these transfection types in two categories: stable (nucleic acid is present for long-term, it affects the progeny of the cell culture) and transient (nucleic acid is present for some or define time). Below we have discussed both types of transfection techniques.