What is RTPCR Test | Book RTPCR Test Online
PCR is one of the most important scientific advances of the 20th century
Polymerase chain reaction (PCR) is a fast and low-cost method for copying small specific DNA or RNA sequences.
Every organism, including plants, animals, bacteria, and viruses, has its own unique nucleic acid sequence. Millions of copies of fragments within these sequences can be made in a short period of time using PCR. It is a novel yet simple method that is a valuable asset in the field of molecular diagnostics.
Even with small amounts of samples, PCR allows for reliable disease diagnosis and monitoring, with high sensitivity that other diagnostic methods, such as culture or serology, may not be able to provide. Many in the diagnostic community regard PCR as the "gold standard" because of its accuracy, precision, and reliability.
Applications of the "DNA photocopier" in the real world 2-6 PCR techniques are an essential component in the arsenal of today's scientists, with applications ranging from diagnostics, research, and prenatal care to agriculture and forensics.
PCR Test is most commonly used to diagnose infections such as influenza, COVID-19, HIV, Chlamydia trachomatis, and viral hepatitis, among others. It has also contributed to the advancement of cervical cancer screening and plays an important role in ensuring the safety of the blood supply.
PCR is also used to enable personalized healthcare beyond initial diagnosis.
measuring a person's viral load (the amount of virus present in a person's body) allows healthcare professionals to assess how well a medicine or treatment is working. This is a critical component of managing certain chronic infections, such as HIV or hepatitis B.
PCR can also be used in liquid biopsy testing (a simple and non-invasive alternative to surgical biopsies) to detect the presence of specific gene mutations (e.g., EGFR and KRAS) in cancers (e.g. lung and colorectal cancer).
PCR is a popular choice in molecular laboratories due to its high sensitivity and broad application. However, other critical complementary technologies, such as next-generation sequencing, have the potential to reveal new information about the role of nucleic acids in disease.
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