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"Our laboratory has developed a fabrication system for functional 3D tissues by stacking cell sheets of confluent cultured cells detached from a temperature-responsive culture dish. Here we describe the protocols for the fabrication of 3D tissues by cell sheet engineering."
Source: Yuji Haraguchi, et al. Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro. Nature Protocols 7 , 850–858 (2012).
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