#wst-8

Cell Counting Kit-8 from Abbkine, Your Best Tool for Cell Toxicity Experiment

Abbkine has developed various cell status check tools for scientific researchers in the field of cytology in past three years. From cell proliferation, cell toxicity to cell apoptosis, you can always find one product suitable for your research project. Today what I recommend is one of Abbkine star product – Cell Counting Kit-8 (Catalogue#KTC011001). Believe me, you could not miss it!

The short name of Cell Counting Kit-8 is CCK-8 Kit, which is established based on WST-8 reaction and widely used for cell proliferation/Toxicity, drug screening as well as tumor drug sensitivity test experiment, etc… Abbkine CCK-8 Kit provides one ready-to-use CCK-8 solution, it is convenient and fast which will be your prefect choice to replace MTT method.

         Figure.1 The reaction mechanism of CCK-8（WST-8）

Compared with traditional method for Cell Toxicity/Proliferation experiment, CCK-8 has many noteworthy benefits as you can find in below chart clearly.

Detection method MTT XTT WST-1 CCK-8 The water solubility of produced formazan Bad (need to add organic solvent to dissolve before detection Good Good Good Product fomat Powder   two solutions Solution one CCK-8 solution Using method Prepare before use Prepare

before use

Ready to use Ready to use Detection sensitivity High Very High Very High High Detection time Relatively longer Relatively shorter Relatively Shorter Shortest Detection wave length 560-600nm 420-480nm 420-480nm 430-490nm Cytotoxicity High Very Low Very Low Very low Reagent stability Normal Relatively poor Normal Very good Batch sample testing Suitable Very suitable Very suitable Very suitable Convenience degree Normal Convenient Very convenient Very convenient

Apart from above mentioned features, Abbkine made several improvements on the formula of its Cell Counting Kit-8, so I believe you can have a pleasant experience on the use of Abbkine CCK-8 Kit including below points:

◎Less toxic, compatible for other downstream assays

◎No organic solvents or isotopes required

◎No harvesting, no washing and no solubilization steps

◎Low toxicity, the same cells can be used for other cell assays

  Insisting in providing high quality and affordable product solution for worldwide scientists, Abbkine deducts almost half of list price than other world renewed CCK-8 Providers. You can see our product specification as below:

Catalogue Number Product Name Size Price KTC011001 Cell Counting Kit-8 (CCK-8) 1ml/5ml/20ml/100ml $20/$64/$205/$718

For the first time purchaser, you are entitled to have some trail discount, and Abbkine also provide simple pack for your pre-experiment use.

Feel free to send your request to [email protected], Abbkine service representative will contact you back shortly.

Here if you want to learn more information about Abbkine cellular assay kits, please review below link:

https://www.abbkine.com/product-category/assay-kit/celluar-assay-kit/.

  About Abbkine Scientific Co., Ltd.

SOD "mi", How to decrypt, Only Abbkine

What is SOD? Both men, women, and children in the fashion industry know: Dabao SOD honey, rich in SOD (superoxide dismutase), is an active substance derived from living organisms, which can eliminate harmful substances produced by the organism during metabolism. Continuous supplementation of SOD to the human body has the special effect of anti-aging. At the same time, as the latest product of Abbkine, I have been adhering to the concept of “affordable, reliable, and practical” to provide the most preferential, convenient and easy-to-use products for scientific researchers, as shown in the figure below. KTB1030 CheKine™ Superoxide Dismutases (SOD) Assay Kit.

So, what’s the secret of SOD antioxidant?

SOD (Superoxide Dismutases, superoxide dismutases) is a metalloenzyme that is widely present in living organisms. It is an important oxygen radical scavenger and can catalyze the disproportionation of superoxide anions to generate H2O2 and O2. SOD is not only a superoxide anion scavenging enzyme, but also a main H2O2 generating enzyme, which plays an important role in biological antioxidant systems. It is widely present in animals, plants, microorganisms and cultured cells, and catalyzes the disproportionation of superoxide anion (O2-) to produce H2O2 and O2. SOD plays a vital role in the body’s oxidation and antioxidant balance. This enzyme can remove superoxide anion free radicals and protect cells from damage.

And SOD is an antioxidant that can slow down skin aging and prevent premature wrinkles and stains on the skin.

Based on this principle, Abbkine designed a new kit : KTB1030 CheKine ™ Superoxide Dismutases (SOD) Assay Kit.

This kit is a blue formazan which is reduced by superoxide anion (O2-) to form nitrogen blue tetrazole. It is a colored substance that can be used for detection; SOD can remove O2- to calculate the SOD content. Superoxide anion (O2-) is derived from xanthine oxidase (XO) -catalyzed reaction. O2- reacts with tetrazolium salt WST-8 to form water-soluble formazan products. SOD removes O2- and is therefore less used for color development reactions. The inhibitory activity of SOD at 450 nm was determined by colorimetry. The activity of SOD is inversely related to the amount of formazan dye produced.

Product name Description Cat NO CheKine™ Superoxide Dismutases (SOD) Assay Kit Detection and quantification of superoxide dismutase KTB1030

  Superoxide dismutase detection kit (KTB1030) advantages and product features:

Compared with NBT (nitro blue tetrazole) method, cytochrome C method, WST-1 method

Produce stable reaction products and higher sensitivity;

SOD activity is measured by absorbance detection at a single time point, suitable for high-throughput screening studies;

The maximum inhibition percentage can be close to 100%, which is not affected by some common interference factors. Many cells and tissue samples contain endogenous hydrogen peroxide, which will interfere with the detection of SOD;

The calculation method is convenient, the linear range is 3.13 -200 U / ml, and the enzyme activity U / ml can be directly calculated through the standard curve;

A wide range of applications: determination of SOD enzyme activity in serum, plasma, tissue / cell lysate and other biological fluids,

The cells are directly lysed without the need for homogenization and the operation is more convenient.

Abbkine focuses on the fields of immunology and cytology, and is committed to innovating and developing various types of antibodies, proteins, analytical reagents and kits, with a view to becoming a key promoter in the fields of life science research and development, and drug development. Here, we present to you the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary and secondary antibodies for immunological experiments; cell research users’ favorite Products ranging from dyes and kits for cell state detection, organelle extraction kits, cell substructure staining and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help you Research career!

About Abbkine Scientific Co., Ltd.

SOD "mi", How to decrypt, Only Abbkine

  What is SOD? Both men, women, and children in the fashion industry know: Dabao SOD honey, rich in SOD (superoxide dismutase), is an active substance derived from living organisms, which can eliminate harmful substances produced by the organism during metabolism. Continuous supplementation of SOD to the human body has the special effect of anti-aging. At the same time, as the latest product of Abbkine, I have been adhering to the concept of “affordable, reliable, and practical” to provide the most preferential, convenient and easy-to-use products for scientific researchers, as shown in the figure below. KTB1030 CheKine™ Superoxide Dismutases (SOD) Assay Kit.

So, what’s the secret of SOD antioxidant?

SOD (Superoxide Dismutases, superoxide dismutases) is a metalloenzyme that is widely present in living organisms. It is an important oxygen radical scavenger and can catalyze the disproportionation of superoxide anions to generate H2O2 and O2. SOD is not only a superoxide anion scavenging enzyme, but also a main H2O2 generating enzyme, which plays an important role in biological antioxidant systems. It is widely present in animals, plants, microorganisms and cultured cells, and catalyzes the disproportionation of superoxide anion (O2-) to produce H2O2 and O2. SOD plays a vital role in the body’s oxidation and antioxidant balance. This enzyme can remove superoxide anion free radicals and protect cells from damage.

And SOD is an antioxidant that can slow down skin aging and prevent premature wrinkles and stains on the skin.

Based on this principle, Abbkine designed a new kit : KTB1030 CheKine ™ Superoxide Dismutases (SOD) Assay Kit.

This kit is a blue formazan which is reduced by superoxide anion (O2-) to form nitrogen blue tetrazole. It is a colored substance that can be used for detection; SOD can remove O2- to calculate the SOD content. Superoxide anion (O2-) is derived from xanthine oxidase (XO) -catalyzed reaction. O2- reacts with tetrazolium salt WST-8 to form water-soluble formazan products. SOD removes O2- and is therefore less used for color development reactions. The inhibitory activity of SOD at 450 nm was determined by colorimetry. The activity of SOD is inversely related to the amount of formazan dye produced.

Product name Description Cat NO CheKine™ Superoxide Dismutases (SOD) Assay Kit 超氧化物歧化酶检测试剂盒 KTB1030

  Superoxide dismutase detection kit (KTB1030) advantages and product features:

Compared with NBT (nitro blue tetrazole) method, cytochrome C method, WST-1 method

Produce stable reaction products and higher sensitivity;

SOD activity is measured by absorbance detection at a single time point, suitable for high-throughput screening studies;

The maximum inhibition percentage can be close to 100%, which is not affected by some common interference factors. Many cells and tissue samples contain endogenous hydrogen peroxide, which will interfere with the detection of SOD;

The calculation method is convenient, the linear range is 3.13 -200 U / ml, and the enzyme activity U / ml can be directly calculated through the standard curve;

A wide range of applications: determination of SOD enzyme activity in serum, plasma, tissue / cell lysate and other biological fluids,

The cells are directly lysed without the need for homogenization and the operation is more convenient.

Abbkine focuses on the fields of immunology and cytology, and is committed to innovating and developing various types of antibodies, proteins, analytical reagents and kits, with a view to becoming a key promoter in the fields of life science research and development, and drug development. Here, we present to you the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary and secondary antibodies for immunological experiments; cell research users’ favorite Products ranging from dyes and kits for cell state detection, organelle extraction kits, cell substructure staining and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help you Research career!

About Abbkine Scientific Co., Ltd.

SOD "mi", How to decrypt, Only Abbkine

  What is SOD? Both men, women, and children in the fashion industry know: Dabao SOD honey, rich in SOD (superoxide dismutase), is an active substance derived from living organisms, which can eliminate harmful substances produced by the organism during metabolism. Continuous supplementation of SOD to the human body has the special effect of anti-aging. At the same time, as the latest product of Abbkine, I have been adhering to the concept of “affordable, reliable, and practical” to provide the most preferential, convenient and easy-to-use products for scientific researchers, as shown in the figure below. KTB1030 CheKine™ Superoxide Dismutases (SOD) Assay Kit.

So, what’s the secret of SOD antioxidant?

SOD (Superoxide Dismutases, superoxide dismutases) is a metalloenzyme that is widely present in living organisms. It is an important oxygen radical scavenger and can catalyze the disproportionation of superoxide anions to generate H2O2 and O2. SOD is not only a superoxide anion scavenging enzyme, but also a main H2O2 generating enzyme, which plays an important role in biological antioxidant systems. It is widely present in animals, plants, microorganisms and cultured cells, and catalyzes the disproportionation of superoxide anion (O2-) to produce H2O2 and O2. SOD plays a vital role in the body’s oxidation and antioxidant balance. This enzyme can remove superoxide anion free radicals and protect cells from damage.

And SOD is an antioxidant that can slow down skin aging and prevent premature wrinkles and stains on the skin.

Based on this principle, Abbkine designed a new kit : KTB1030 CheKine ™ Superoxide Dismutases (SOD) Assay Kit.

This kit is a blue formazan which is reduced by superoxide anion (O2-) to form nitrogen blue tetrazole. It is a colored substance that can be used for detection; SOD can remove O2- to calculate the SOD content. Superoxide anion (O2-) is derived from xanthine oxidase (XO) -catalyzed reaction. O2- reacts with tetrazolium salt WST-8 to form water-soluble formazan products. SOD removes O2- and is therefore less used for color development reactions. The inhibitory activity of SOD at 450 nm was determined by colorimetry. The activity of SOD is inversely related to the amount of formazan dye produced.

Product name Description Cat NO CheKine™ Superoxide Dismutases (SOD) Assay Kit 超氧化物歧化酶检测试剂盒 KTB1030

  Superoxide dismutase detection kit (KTB1030) advantages and product features:

Compared with NBT (nitro blue tetrazole) method, cytochrome C method, WST-1 method

Produce stable reaction products and higher sensitivity;

SOD activity is measured by absorbance detection at a single time point, suitable for high-throughput screening studies;

The maximum inhibition percentage can be close to 100%, which is not affected by some common interference factors. Many cells and tissue samples contain endogenous hydrogen peroxide, which will interfere with the detection of SOD;

The calculation method is convenient, the linear range is 3.13 -200 U / ml, and the enzyme activity U / ml can be directly calculated through the standard curve;

A wide range of applications: determination of SOD enzyme activity in serum, plasma, tissue / cell lysate and other biological fluids,

The cells are directly lysed without the need for homogenization and the operation is more convenient.

Abbkine focuses on the fields of immunology and cytology, and is committed to innovating and developing various types of antibodies, proteins, analytical reagents and kits, with a view to becoming a key promoter in the fields of life science research and development, and drug development. Here, we present to you the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary and secondary antibodies for immunological experiments; cell research users’ favorite Products ranging from dyes and kits for cell state detection, organelle extraction kits, cell substructure staining and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help you Research career!

About Abbkine Scientific Co., Ltd.

Quantitative determination of LDH in samples, Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit will offer you an easier method

Lactate dehydrogenase (LDH) is an oxidoreductase found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvate and back, as it converts NAD+ to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another.

Quantification of Lactate dehydrogenase has clinical significance because serum levels of certain lactate dehydrogenase isoenzymes reflect the pathological conditions of specific tissues. When tissue is damaged by disease, injury, or toxic substances, lactate dehydrogenase is released into the bloodstream. Because lactate dehydrogenase is a fairly stable enzyme, it has been widely used to evaluate tissue, cell damage and toxicity.

Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit, more simple, convenient and sensitive

Product Name CAT # CheKine™ Lactate Dehydrogenase Assay Kit KTB1110

【Principle】

provides a simple and easy colorimetric assay for measuring LDH in samples. In this colorimetric assay, LDH reduces NAD to NADH, which then interacts with a probe to produce a color (λmax = 450 nm), the LDH present in the sample is proportional to the signal obtained.

【Samples】

serum, plasma, cell culture supernatants, tissue/cell lysates, culture medium, fermentation and other biological fluids.

【Kit components】

Name Storage temperature Function Assay Buffer 4ºC maintain reaction environment Lactate -20ºC LDH substrate NAD -20ºC LDH substrate WST-8 -20ºC, protect from light coloration Enhancer -20ºC, protect from light electron transport complex LDH Standard -20ºC Prepare standard curve

【Procedure】

Working Reagent Preparation

Add standard and sample per well, then add Working Reagent to each well. Tap plate to mix. Immediately read optical density at 450nm (OD0).

Incubate for 30 min at 37°C in the dark. Read optical density at 450nm again (OD30).

【Standard Curve】

About Abbkine Scientific Co., Ltd.

New Post has been published on https://www.abbkine.com/abbkine-chekine-lactate-dehydrogenase-ldh-assay-kit/

Quantitative determination of LDH in samples, Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit will offer you an easier method

Lactate dehydrogenase (LDH) is an oxidoreductase found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvate and back, as it converts NAD+ to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another.

Quantification of Lactate dehydrogenase has clinical significance because serum levels of certain lactate dehydrogenase isoenzymes reflect the pathological conditions of specific tissues. When tissue is damaged by disease, injury, or toxic substances, lactate dehydrogenase is released into the bloodstream. Because lactate dehydrogenase is a fairly stable enzyme, it has been widely used to evaluate tissue, cell damage and toxicity.

Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit, more simple, convenient and sensitive

Product Name CAT # CheKine™ Lactate Dehydrogenase Assay Kit KTB1110

【Principle】

provides a simple and easy colorimetric assay for measuring LDH in samples. In this colorimetric assay, LDH reduces NAD to NADH, which then interacts with a probe to produce a color (λmax = 450 nm), the LDH present in the sample is proportional to the signal obtained.

【Samples】

serum, plasma, cell culture supernatants, tissue/cell lysates, culture medium, fermentation and other biological fluids.

【Kit components】

Name Storage temperature Function Assay Buffer 4ºC maintain reaction environment Lactate -20ºC LDH substrate NAD -20ºC LDH substrate WST-8 -20ºC, protect from light coloration Enhancer -20ºC, protect from light electron transport complex LDH Standard -20ºC Prepare standard curve

【Procedure】

Working Reagent Preparation

Add standard and sample per well, then add Working Reagent to each well. Tap plate to mix. Immediately read optical density at 450nm (OD0).

Incubate for 30 min at 37°C in the dark. Read optical density at 450nm again (OD30).

【Standard Curve】

About Abbkine Scientific Co., Ltd.

New Post has been published on https://www.abbkine.com/abbkine-chekine-lactate-dehydrogenase-ldh-assay-kit/

Quantitative determination of LDH in samples, Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit will offer you an easier method

Lactate dehydrogenase (LDH) is an oxidoreductase found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvate and back, as it converts NAD+ to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another.

Quantification of Lactate dehydrogenase has clinical significance because serum levels of certain lactate dehydrogenase isoenzymes reflect the pathological conditions of specific tissues. When tissue is damaged by disease, injury, or toxic substances, lactate dehydrogenase is released into the bloodstream. Because lactate dehydrogenase is a fairly stable enzyme, it has been widely used to evaluate tissue, cell damage and toxicity.

Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit, more simple, convenient and sensitive

Product Name CAT # CheKine™ Lactate Dehydrogenase Assay Kit KTB1110

【Principle】

provides a simple and easy colorimetric assay for measuring LDH in samples. In this colorimetric assay, LDH reduces NAD to NADH, which then interacts with a probe to produce a color (λmax = 450 nm), the LDH present in the sample is proportional to the signal obtained.

【Samples】

serum, plasma, cell culture supernatants, tissue/cell lysates, culture medium, fermentation and other biological fluids.

【Kit components】

Name Storage temperature Function Assay Buffer 4ºC maintain reaction environment Lactate -20ºC LDH substrate NAD -20ºC LDH substrate WST-8 -20ºC, protect from light coloration Enhancer -20ºC, protect from light electron transport complex LDH Standard -20ºC Prepare standard curve

【Procedure】

Working Reagent Preparation

Add standard and sample per well, then add Working Reagent to each well. Tap plate to mix. Immediately read optical density at 450nm (OD0).

Incubate for 30 min at 37°C in the dark. Read optical density at 450nm again (OD30).

【Standard Curve】

About Abbkine Scientific Co., Ltd.

New Post has been published on https://www.abbkine.com/abbkine-chekine-lactate-dehydrogenase-ldh-assay-kit/

Quantitative determination of LDH in samples, Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit will offer you an easier method

Lactate dehydrogenase (LDH) is an oxidoreductase found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvate and back, as it converts NAD+ to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another.

Quantification of Lactate dehydrogenase has clinical significance because serum levels of certain lactate dehydrogenase isoenzymes reflect the pathological conditions of specific tissues. When tissue is damaged by disease, injury, or toxic substances, lactate dehydrogenase is released into the bloodstream. Because lactate dehydrogenase is a fairly stable enzyme, it has been widely used to evaluate tissue, cell damage and toxicity.

Abbkine CheKine™ Lactate Dehydrogenase (LDH) Assay Kit, more simple, convenient and sensitive

Product Name CAT # CheKine™ Lactate Dehydrogenase Assay Kit KTB1110

【Principle】

provides a simple and easy colorimetric assay for measuring LDH in samples. In this colorimetric assay, LDH reduces NAD to NADH, which then interacts with a probe to produce a color (λmax = 450 nm), the LDH present in the sample is proportional to the signal obtained.

【Samples】

serum, plasma, cell culture supernatants, tissue/cell lysates, culture medium, fermentation and other biological fluids.

【Kit components】

Name Storage temperature Function Assay Buffer 4ºC maintain reaction environment Lactate -20ºC LDH substrate NAD -20ºC LDH substrate WST-8 -20ºC, protect from light coloration Enhancer -20ºC, protect from light electron transport complex LDH Standard -20ºC Prepare standard curve

【Procedure】

Working Reagent Preparation

Add standard and sample per well, then add Working Reagent to each well. Tap plate to mix. Immediately read optical density at 450nm (OD0).

Incubate for 30 min at 37°C in the dark. Read optical density at 450nm again (OD30).

【Standard Curve】

About Abbkine Scientific Co., Ltd.