Macro invertebrates are the organisms of 0.2 mm in size or more and can be seen with naked eye. They may consist of predators, preys or pests which play a significant role in balancing the natural agro-ecosystem. The organisms were sampled from wheat-weed crop complex of Baghdad-ul-Jadeed university campus and major cropland area of district Bahawalpur. Overall the OrderColeoptera was dominant followed by Hemiptera and Orthopterawhile order Opisthopora, Odonata, Lepidoptera, Hymenoptera and Zygentomawere represented by single species each. Direct hand picking, hand nets and automated sifter techniques were employed for sampling of fauna. Wisemen solution was used for preservation and their systematics was determined withtaxonomic literature. The economic status was also determined to separate different categories. Such results are valuable contribution for ecological management of wheat agro-ecosystem.