#nanopore

Chip In

Early detection is often critical to surviving cancer, making methods of diagnosis a major focus for innovation. Pancreatic ductal adenocarcinoma (PDCA), a type of pancreatic cancer, is often detected too late, so researchers have developed a new diagnostic tool for this disease. Known as a nanoplasmic sensor assay, the test is designed to spot small markers of cancer in blood plasma. In this case, it identifies tumour-derived extracellular vesicles (tEVs), minute cytoplasmic sacs produced by cells, containing proteins and other molecules characteristic of tumours. The test involves shining a beam of light on a tiny chip, with an array of nanoscale holes, or nanopores (pictured), coated in antibodies specific to PDCA tEVs. Binding of tEVs (shown as multicoloured spheres) to these antibodies causes a shift in the light re-emitted from the chip, enabling detection. Highly sensitive and accurate, this assay should greatly improve our ability to diagnose pancreatic cancer.

Written by Emmanuelle Briolat

Image by Hyungsoon Im, Massachusetts General Hospital, Boston, MA, USA

Center for Systems Biology, Massachusetts General Hospital, Boston, MA, USA

Image copyright held by the original authors

Research published in Science Translational Medicine, May 2017

Nanopore Sequencing for Research & Diagnostics

Overview

Quintara provides rapid, cost-effective Nanopore-based sequencing services—simply submit your plasmid DNA and receive results in just one day. This swift turnaround empowers you to quickly access critical data and advance your projects. With advanced technology and a commitment to quality, Quintara ensures fast, reliable sequencing solutions you can trust.

Promotion

Offer:

    1. AmpValue: 5 Free Samples (Promo Code: AmpValue5Free)

    2. Amplicon Sequencing Clean Up: $4 OFF >> Only $1/Sample (Promo Code: Cleanup1)

    3. AmpStandard: $7+ OFF >> From $7.99/Sample (Promo Code: FriAmpS)

        AmpPro: $10+ OFF >> From $19.99/Sample (Promo Code: FriAmpPro)

        WPS Standard: $7+ OFF >> From $7.99/Sample (Promo Code: FriWPSS)

Terms and Conditions:

•  The promotion 1 applies to all customers ordering 1st Nanopore Amplicon Sequencing service, valid until May 31.

•  The promotion 2 applies to all Nanopore Amplicon Sequencing services (AmpValue / AmpStandard / AmpPro).

•  The promotion 3 applies to all qualifying every Friday orders placed starting May 1, based on ET.

•  Only one promo code may be applied per order. The system will automatically select and apply the best available discount for your order. You may manually adjust the applied discount code at your discretion if preferred.

•  The discount will be applied automatically when you place your order through the QuinGo online portal.

•  Quintara Biosciences reserves all rights to this promotion.

Results ready for download the next day

Affordable, fast and accurate

Simply submit your sample

Third-generation sequencing technology

Technology

In nanopore sequencing, DNA is passed through tiny pores embedded in a membrane. Each base is identified by how it alters the ionic current as it moves through the pore. Because this method can generate ultra-long reads, it’s particularly effective for sequencing large or complex regions—such as plasmids, large insertions, structural variations, and repeat-rich sequences.

Workflow

Experience a seamless workflow with Quintara: simply send us your DNA samples, and we'll prepare them into a sequencing library. The library is then sequenced on the Oxford Nanopore platform, generating long reads that are used to assemble your sequence of interest.

01

Sample Submission

02

Library Preparation

03

Sequencing

04

Data Analysis

Services

Whole Plasmid Sequencing Services

WPS Express

WPS Standard

WPS Pro

Cost-effective sequencing for non-complex plasmids. Ideal for high-throughput construct screening.

The industry standard for confirming plasmid sequence and quality. Ideal for routine plasmid verification.

Deep sequencing with full-length reads for resolving plasmid mixtures. Ideal for plasmid library verification.

Amplicon Sequencing Services

AmpValue undefined

AmpStandard

AmpPro

Budget-friendly option for sequencing short to medium-length amplicons. Ideal for high-throughput projects.

Classic long-read sequencing for clonal amplicons of any length. Ideal for routine amplicon verification.

Deep sequencing with full-length reads for resolving amplicon mixtures.  Ideal for amplicon library verification.

Service Guidelines

Same-Day Service

Data will be delivered by 4 PM the same day if the samples are submitted before 10 AM (Eastern Time). Now available in the Cambridge and Boston areas.

Overnight Sequencing

If your location is near our Quintara laboratories (CO, TX, CA, MA, CT, DC),

you can enjoy:

•  Free sample pickup by our courier from drop boxes

•  Overnight sequencing with next-morning report delivery

24-Hour Data Access

Data available within 24 hours after samples are received by our CA or MA laboratories.

FAQ

How do I submit my samples for nanopore sequencing?

Once your order is successfully placed through the Quintara website, please print the order submission form, fold it so that the barcode is visible, and place it in a small bag with your samples. Then, deposit the bag into your nearest Quintara drop box, and our team will take care of the rest.

•  For labeling your PCR tubes, please clearly mark the first tube of the strip with your initials followed by the last two digits of your Order ID. For example, if your initials are YZ and the Order ID ends in 42, label the first tube as YZ42. The second tube should be labeled as 2, the eighth tube as 8, and so on. Please refer to the image below for the correct labeling format. Do not write the actual sample names on the tubes.

•  When submitting samples using 96-well plates, please ensure that there are no empty wells between samples, whether you are submitting by row or column. If using column numbering, label the first column (A01 as sample #1, H01 as sample #8). If using row numbering, label the first row (A01 as sample #1, A12 as sample #12).

What is the typical turnaround time for your Nanopore Sequencing service?

Our Nanopore sequencing service offers next-morning data delivery, with results typically available by 9am the following day. If you have not received your data by this time, please contact us promptly by emailing [email protected]. This will allow us to provide an update and resolve any potential issues, ensuring no further delays in receiving your sequencing results. We operate 7 days a week to support your needs.

How do I get in touch with support if I have questions about my order or need help with my data?

If you have any questions about your order or need assistance with your data, the best way to reach us is by emailing [email protected]. Our support team is ready to assist you with any inquiries or issues you may have.

What steps should I take if I need to cancel or modify my order after it has been placed?

If you need to cancel or modify your order after it has been placed, please contact us as soon as possible via email at [email protected]. We understand that circumstances may change and will do our best to accommodate your request. If the order is still in the early stages of processing, we may be able to make adjustments. However, once sequencing has begun, changes may not be possible. Please include your numeric order ID in the email for quicker assistance.

How can I request additional services after verifying the sequencing results, such as sample returns or DNA preparation from positive clones?

To request additional services, such as sample returns or DNA preparation from positive clones, please contact us via email at [email protected]. Provide your order details and specify the services you require, and we will assist you with the next steps.

How do I know if my sequencing results are ready for download, and how will I be notified?

You will receive an automatic email with a download link as soon as your sequencing data is uploaded to our website portal. You can either download the data directly from the link in the email or log into your Quintara account and access the data from the portal.

DNA holds the instructions that make us who we are. If we want to read those instructions, say to understand our predisposition to genetic disorders, we need to carefully ‘unzip’ DNA’s iconic double-helix structure. This is neither easy nor fast, and the process involves intense heating and chemicals that can damage the DNA. Now, in a new study published in ACS Nano, a research team led by The…

Artificial Eater🧬🍽️

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Los dibujos de Leonardo da Vinci tienen microbiomas únicos, encuentra un estudio

Este reciente hallazgo podría permitir ayudar a frenar el deterioro de las obras de arte envejecidas y a detectar posibles falsificaciones.