-Dry the wets

-Purple the drys

-Wet the purples

-Brown the wets

-Wet the browns

-Clear the wets

-Wet the clears/purples

-Pink the wets

-Wet the pinks/purples

-Dry the wets

The Gram stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram. It is one of the most useful staining procedures because it classifies bacteria into two large groups: gram-positive and gram-negative.

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Different types of Microbial Staining Techniques

Bacteria can be studied either in unstained (wet mount) or stained preparation. By staining any bacterial cell, we can easily identify its cell shape and other easily locate them under the microscope. Different type of staining methods has been evolved time to time to study bacterial cells / cells under the microscope. 

Wet mount preparation: 

Wet mount preparation is useful for the…

Peptidoglycan Structure and Functions

Peptidoglycan, also known as murein, is a polymer consisting of sugar and amino acids that forms a mesh – like layer outside the plasma membrane of most bacteria, forming the cell wall. 

The peptide chain can be cross linked to the peptide chain of another stand forming the 3D mesh like layer. Peptidoglycan serves a structural role in the bacterial cell wall, giving structural strength as well as…