I had set aside most of my day to walk this grad student from another lab through our sequencing prep protocol, but when she made her sample dilutions she ended up using a plate made for 100 ul samples unstead of 200 ul, so the liquid was partially touching the plate seal in almost every well 😭 very understandable mistake but I had to push past my often-misguided urge to just go ahead and see if it works and be like...ok actually this is a time-consuming protocol that uses expensive reagents and if there's cross-sample contamination at this step we will literally never be able to untangle it completely. so. go do the dilutions again and we'll try another day.

Responsibility begins with the willingness to acknowledge oneself as cause in any situation. This precludes blame, shame, guilt, resentment and remorse. It is always false, in a larger sense, to blame another person for one's own troubles or to feel guilt over perceived self-blame. Misperceptions and misinterpretations of human interactions lead to false decisions, assumptions, conclusions and judgments.

speedrunning cause i need to wash my hair, shave, do my nails, and figure out who tf i'm gonna vote tomorrow all in the next 2h

but I don't want to be reasonable. I want to stay up all night and download Sandman at 4am, then binge watch it until 9am when I have to go work in the 100 degree heat and possibly some rain after eating nothing but a bologna sandwich on stale bread that I bought for $1.29.

wait wtf we've been on a mask mandate for a week and i had no idea?????????