World is rich in knowledge with a wide diversity of medicinal plants which provide people with traditional healing methods for diseases in general healthcare area. The agar diffusion method was used to examine the antibacterial activity of different solvent extracts of Biophytum sensitivum tested against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, and Staphylococcus aureus. For qualitative phytochemical investigation, several solvent extracts of the complete Biophytum sensitivum plant were investigated. The minimum inhibitory concentration (MIC) of Biophytum sensitivum ethanol extract had significant antimicrobial effectiveness against Escherichia coli (456 µg/ml), and Pseudomonas aeruginosa (419 µg/ml). The ethanolic extract showed (419 µg/ml and 398 µg/ml) antimicrobial activity against Klebsiella pneumonia and Staphylococcus aureus respectively. The lowest antibacterial activity was found in acetone extract with MIC (198µg/ml) of Biophytum sensitivum. Various parts of the plant has the presence of variety of phytoconstituents such as flavonoids, saponins, tannins, terpenes, steroids, amino acids, essential oil, polysaccharides and pectin.
